protein identification -凯发天生赢家一触即发官网

protein identification


service description

modern liquid chromatography and mass spectrometry technology provides a means to routinely perform non-targeted protein identification¹. bgi can help simplify the challenge of protein identification for your project.

project workflow

in-gel identification

we can identify proteins isolated in gel bands or spots. our service includes destaining, in-gel digestion, nano-flow lc-ms/ms, and peptide spectral match data analysis.
  • in-gel digestion
  • lc-ms/ms analysis
  • data analysis

in-solution identification

our in-solution protein identification services can be used for applications such as monitoring recombinant protein expression and affinity purification systems.
  • in-solution digestion
  • lc-ms/ms analysis
  • data analysis


[1]. baldwin ma. protein identification by mass spectrometry: issues to be considered. mol cell proteomics. 2004 jan;3(1):1-9. epub 2003 nov 6. review. pubmed pmid: 14608001

mass spectrometry service specification

protein identification services are performed using nano-flow liquid chromatography and high resolution orbitrap mass spectrometry.
  • sample preparation and services

    • digestion performed using sequencing-grade trypsin
    • 60 min nano-flow lc-ms/ms using a q exactive hf-x orbitrap mass spectrometer
  • mass spec services quality standard

    • summary including all methods and data analysis
    • reports provided in excel or pdf format, raw files available upon request
  • turn around time

    • typical 2-3 weeks from sample qc acceptance to data report delivery
    • prime expedited services with 1 week turn around time is available

sample requirements

we accept protein samples in solution or gel format. for complex in-solution samples we can provide electrophoresis services, including preparative sds-page and analytical staining.
sample typeamount and concentrationminimum sample volume
protein sample in liquid solution or gel band/spotrecommended 100 µg; 2 µg/µl or above10 µl

data analysis

advanced bioinformatics
  • data analysis and validation performed with sequest or mascot
  • top protein identifications, peptide spectral match count, and sequence coverage provided
  • up to 30 proteins ranked by peptide spectral match count