proteome profiling -凯发天生赢家一触即发官网

proteome profiling


service description

proteome profiling analysis refers to the analysis of an entire proteome from complex samples like complete cells, tissues and body fluids. it is most often used to identify as many peptides and proteins as possible. proteome profiling analysis based on mass spectrometry (ms) can provide reference information for high-throughput quantitative proteomics and protein modification analysis.

project workflow

proteome profiling

proteome profiling is a cost effective, high value solution for monitoring hundreds-to-thousands of proteins simultaneously. we perform sample digestion using sequencing-grade trypsin or alternative proteolytic method, and then trypsin-digested peptide samples are analyzed using nano-flow lc-ms/ms. the spectral counting method provides information on protein relative abundances¹. besides, our hplc sample fractionation systems are highly recommended for complex sample types to reduce sample complexity and maximize protein sequence coverage and detection dynamic range².
  • sample preparation
  • lc fractionation
  • lc-ms/ms analysis
  • data analysis


[1] zhang, y., et al., tissue-based proteogenomics reveals that human testis endows plentiful missing proteins. j proteome res. 2015 sep 4;14(9):3583-94.  [2] chandramouli k, qian py. proteomics: challenges, techniques and possibilities to overcome biological sample complexity. hum genomics proteomics. 2009 dec 8;2009:239204. 

service specification

  • sample preparation and services

    • digestion performed using sequencing-grade trypsin
    • off-line sample fractionation using hplc technology
    • each fraction analyzed using nano-flow lc-ms/ms
    • peptide spectral match (psm) data utilized for constructing proteomic profile
  • quality standard

    • summary includes all methods and data analysis
    • reports provided in excel or pdf format, raw files available upon request
  • turn around time

    • typical 4-5 weeks from sample qc acceptance to data report delivery

sample requirements

sample typeamount and concentration minimum sample volume
protein sample in liquid solution or in gel band/spot recommended 100 µg for each enzyme: 2 µg/µl100 µl for each enzyme
protein sample in liquid solution or in gel band/spot minimum required 10 µg for each enzyme: 1 µg/µl10 µl for each enzyme

data analysis

advanced bioinformatics
  • data analysis and validation performed with mascot
  • go (gene ontology) category analysis
  • cog (cluster of orthologous groups of proteins) category analysis
  • pathway analysis