single cell sequencing, fast & accurate -凯发天生赢家一触即发官网

single cell sequencing-bgi

single cell sequencing

single cell sequencing is used for the study of heterogeneous samples, rare cell types, cell lineage relationships, and disease evolution. we offer high quality, affordable single cell services, with world-class support from our highly experienced single cell project experts.

quick contact

single cell sequencing introduction

single cell sequencing is commonly applied to solve problems associated with the study of heterogeneous samples, rare cell types, cell lineage relationships, and disease evolution. by using deep sequencing of dna and rna from a single cell, cellular functions of individual cells can be investigated in great detail.


bgi applies the superior method of multiple displacement amplification (mda) for single cell genomic dna amplification (wgs/wes), and switching mechanism at 5′ end of rna template (smart-seq2) for single cell rna amplification (transcriptome/rna quantification). contact us with your project requirements, our experts are here to help.

experience of 1000s of single cell projects, our experts are here to ensure your success.

guaranteed data quality at competitive pricing enabled by proprietary technology.

rapid turnaround times and expert support, no project too big or small

single cell sequencing-bgi service overview

sample requirements

dna

single cell: 1-2 cells - 4μl pbs buffer

a few cells: 2-1000 cells - 4μl pbs buffer

amplified single cell gdna/cdna:

concentration: >20ng/ μl, quantity: >3μg


rna

single cell: 1-2 cells - 4μl lysis buffer

a few cells: 2-1000 cells - 4μl lysis buffer

amplified single cell gdna/cdna:

concentration: >0.2ng/μl, quantity: >1ng

total rna for low input library:

concentration: >50pg/μl, quantity: >2ng;

rna 28s/18s ≥1,rin ≥7

sequencing standards

pe100 sequencing option, depending on your application.


guaranteed ≥90% of bases with quality score of ≥q20.


cell sorting by mouth-controlled microcapillary pipetting system.

key service details

• typical 40 working days from sample qc acceptance to filtered raw data availability.

• expedited services are available, contact your local bgi specialist for details.

• reports and output data files are delivered in industry standard file formats: bam, .xls, .png and fastq data.

download service overview

dnbseq™ technology platforms

bgi's single cell sequencing services are typically executed with proprietary dnbseq™ sequencing technology platforms, for great sequencing data at some of the lowest costs in the industry. dnbseq™ can offer advantages in terms of lower amplification error rates and much lower duplication rates for some sequencing applications. in addition, studies have shown the lower index hopping rate in dnbseq™ platforms.

contact us with any general enquiries or questions for our sequencing experts, we are here to help.

to submit your enquiry, you must check the "i agree …" box for the privacy policy.

access further sequencing resources

we care for your samples from the start through to the result reporting. highly experienced laboratory professionals follow strict quality procedures to ensure the integrity of your results. access more sequencing resources below for full access to our range of educational resources and sample guides.

register for a bgi account

opening a bgi customer account enables you to request a quote and order services from us. it also provides access to our range of educational resources including educational webinars providing practical sequencing tips and our complete sample preparation guides.

top

网站地图